| Peer-Reviewed

Effect of Hydroalcoholic Extract of Stem Bark of Bridelia ferruginea on Blood Coagulation

Received: 1 April 2022     Accepted: 12 May 2022     Published: 24 May 2022
Views:       Downloads:
Abstract

Bridelia ferruginea is a plant commonly used in several regions of the world for its various properties. It is offered to certain patients with cerebrovascular accidents. It is with this in mind that this study is undertaken in order to verify whether B. ferruginea has an activity on blood coagulation. Materiel and method: Stem bark of B. ferruginea was collected at Noepe (24 km northwest of Lome). Hydroalcoholic extract (50/50) was obtained by evaporation. Male Wistar rats were used for the tests. Qualitative screening was done according to conventional methods and total phenols were quantified. Some parameters of coagulation (blood platelets, aPTT, PR and fibrinogen) were determined. Oxidative stress was induced by Fe-NTA. Results were analysed by GraphPad®Prism 8.4.2. Analysis of variance (ANOVA) was used to compare several groups. The difference between groups was determined by Tukeyʼs test and considered significant at p<0.05. Results: Hydroalcoholic extract of B. ferruginea increased aPTT and decreased PR. Compared to the negative control (distilled water), oxidative stress was responsible in vivo for an increase in platelets blood number and PR and a decrease in aPTT and fibrinogen. The hydroalcoholic extract of B. ferruginea caused a decrease in platelets blood number (at low concentration), PR and fibrinogen level and an increase in the platelets blood number (at high concentration) and aPTT. Hydroalcoholic extract of B. ferruginea decreased MDA levels and increased GSH levels in rats subjected to oxidative stress. Conclusion: The present study proved existence of antioxidant and anticoagulant activities of B. ferruginea stem bark. There is a link between oxidative stress and coagulation. Future studies may better elucidate interaction between oxidative stress and coagulation mechanisms involved.

Published in Journal of Diseases and Medicinal Plants (Volume 8, Issue 2)
DOI 10.11648/j.jdmp.20220802.14
Page(s) 41-45
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2022. Published by Science Publishing Group

Keywords

Bridelia ferruginea, Oxidative Stress, Blood Coagulation

References
[1] WHO. Final report of the first meeting of the WHO regional expert committee on traditional medicine. Harare, Zimbabwe. 2001.
[2] Chevallier A. Encyclopedia of medicinal plants: identification, preparation, care. 2nd ed. Limited E, editor. Dorling Kindersiey Limited, editor; 2001. 335p.
[3] Kombate D, Assogba K, Kumako V, Diatewa J, Apetse K, Guinhouya KM, et al. Hospital prevalence of cerebral venous thrombosis and risk factors in Togo. African J Neurol Sci. 2017; 36 (1): 23-29. eISSN: 1015-8618.
[4] Kabesh K, Senthilkumar P, Ragunathan R, Kumar RR. Phytochemical Analysis of Catharanthus roseus Plant Extract and its Antimicrobial Activity. Int J Pure Appl Biosci. 2015; 3 (2): 162–72. ISSN: 2320 – 7051.
[5] Shetty K, Curtis OF, Levin RE, Witkowsky R, Ang W. Prevention of Vitrification Associated with in vitro Shoot Culture of Oregano. (Origanum vulgare) by Pseudomonas spp. J Plant Physiol. 1995; 147 (3–4): 447–51. https://doi.org/10.1016/S0176-1617(11)82181-4
[6] Kagnou, H., Simalou, O., Tchani, G., Sanvee, S., Agbodan, K. A., Toundou, O., & Kpegba K. Phytochemical study and comparative antioxidant activity of the three varieties of Catharanthus roseus (L.) G. Don. International J Biol Chem Sci. 2020; 14 (6): 2352–61. eISSN: 1997-342X.
[7] Khan N, Sultana S. Inhibition of two stage renal carcinogenesis, oxidative damage and hyperproliferative response by Nigella sativa. Eur J Cancer Prev. 2005; 14 (2): 159–68. doi: 10.1097/00008469-200504000-00012.
[8] Patlolla AK, Barnes C, Yedjou C, Velma VR TP. Oxidative stress, DNA damage, and antioxidant enzyme activity induced by hexavalent chromium in Sprague-Dawley rats. Env Toxicol. 2009; 24 (1): 66–73. doi: 10.1002/tox.20395.
[9] Sedlak J and Lindsay RH. Estimation of Total Protein-Bound, and Nonprotein Sulfhydryl Groups in Tissue with Ellman’s Reagent. Anal Biochem. 1968; 25: 1192–205. doi: 10.1016/0003-2697(68)90092-4.
[10] Amiot MJ, Riollet C, Landrier JF. Polyphénols et syndrome métabolique: Polyphenols and metabolic syndrome. Médecine des Mal Métaboliques. 2009 Nov 1; 3 (5): 476–82. Doi: MMM-11-2009-3-5-1957-2557-101019-200907501.
[11] Bidie ADP, Bahi G, Meite S, Yapo AF, N’guessan JD, and Djaman AJ. Evaluation of the antioxidant activity of total aqueous extract of Bridelia ferruginea benth. (Euphorbiaceae). World J Pharm Sci. 2015; 3 (10): 2125–34. https://wjpsonline.com/index.php/wjps/article/view/antioxidant-activity-bridelia-ferruginea-benth-euphorbiaceae
[12] Salvemini D. Cuzzocrea S. Oxidative stress in septic shock and disseminated intravascular coagulation. Free Radic Biol Med. 2002; 33 (9): 1173–85. doi: 10.1016/s0891-5849(02)00961-9.
[13] Pawlak K, Borawski J, Naumnik B, Mysliwiec M. Relationship between oxidative stress and extrinsic coagulation pathway in haemodialyzed patients. Thromb Res. 2003 Mar 15; 109 (5–6): 247–51. doi: 10.1016/s0049-3848(03)00241-x.
[14] Danziger J. Vitamin K-dependent proteins, warfarin, and vascular calcification. Clin J Am Soc Nephrol. 2008 Sep; 3 (5): 1504–10. doi: 10.2215/CJN.00770208.
Cite This Article
  • APA Style

    Messanh Delagnon Irenee Kueviakoe, Sabrina Chris Janiba Sanvee, Hezouwe Magnang, Komlan Mawubédjro Dossou-Yovo, Aboudoulatif Diallo, et al. (2022). Effect of Hydroalcoholic Extract of Stem Bark of Bridelia ferruginea on Blood Coagulation. Journal of Diseases and Medicinal Plants, 8(2), 41-45. https://doi.org/10.11648/j.jdmp.20220802.14

    Copy | Download

    ACS Style

    Messanh Delagnon Irenee Kueviakoe; Sabrina Chris Janiba Sanvee; Hezouwe Magnang; Komlan Mawubédjro Dossou-Yovo; Aboudoulatif Diallo, et al. Effect of Hydroalcoholic Extract of Stem Bark of Bridelia ferruginea on Blood Coagulation. J. Dis. Med. Plants 2022, 8(2), 41-45. doi: 10.11648/j.jdmp.20220802.14

    Copy | Download

    AMA Style

    Messanh Delagnon Irenee Kueviakoe, Sabrina Chris Janiba Sanvee, Hezouwe Magnang, Komlan Mawubédjro Dossou-Yovo, Aboudoulatif Diallo, et al. Effect of Hydroalcoholic Extract of Stem Bark of Bridelia ferruginea on Blood Coagulation. J Dis Med Plants. 2022;8(2):41-45. doi: 10.11648/j.jdmp.20220802.14

    Copy | Download

  • @article{10.11648/j.jdmp.20220802.14,
      author = {Messanh Delagnon Irenee Kueviakoe and Sabrina Chris Janiba Sanvee and Hezouwe Magnang and Komlan Mawubédjro Dossou-Yovo and Aboudoulatif Diallo and Ahoefa Vovor and Kwashie Eklu-Gadegbeku},
      title = {Effect of Hydroalcoholic Extract of Stem Bark of Bridelia ferruginea on Blood Coagulation},
      journal = {Journal of Diseases and Medicinal Plants},
      volume = {8},
      number = {2},
      pages = {41-45},
      doi = {10.11648/j.jdmp.20220802.14},
      url = {https://doi.org/10.11648/j.jdmp.20220802.14},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jdmp.20220802.14},
      abstract = {Bridelia ferruginea is a plant commonly used in several regions of the world for its various properties. It is offered to certain patients with cerebrovascular accidents. It is with this in mind that this study is undertaken in order to verify whether B. ferruginea has an activity on blood coagulation. Materiel and method: Stem bark of B. ferruginea was collected at Noepe (24 km northwest of Lome). Hydroalcoholic extract (50/50) was obtained by evaporation. Male Wistar rats were used for the tests. Qualitative screening was done according to conventional methods and total phenols were quantified. Some parameters of coagulation (blood platelets, aPTT, PR and fibrinogen) were determined. Oxidative stress was induced by Fe-NTA. Results were analysed by GraphPad®Prism 8.4.2. Analysis of variance (ANOVA) was used to compare several groups. The difference between groups was determined by Tukeyʼs test and considered significant at pResults: Hydroalcoholic extract of B. ferruginea increased aPTT and decreased PR. Compared to the negative control (distilled water), oxidative stress was responsible in vivo for an increase in platelets blood number and PR and a decrease in aPTT and fibrinogen. The hydroalcoholic extract of B. ferruginea caused a decrease in platelets blood number (at low concentration), PR and fibrinogen level and an increase in the platelets blood number (at high concentration) and aPTT. Hydroalcoholic extract of B. ferruginea decreased MDA levels and increased GSH levels in rats subjected to oxidative stress. Conclusion: The present study proved existence of antioxidant and anticoagulant activities of B. ferruginea stem bark. There is a link between oxidative stress and coagulation. Future studies may better elucidate interaction between oxidative stress and coagulation mechanisms involved.},
     year = {2022}
    }
    

    Copy | Download

  • TY  - JOUR
    T1  - Effect of Hydroalcoholic Extract of Stem Bark of Bridelia ferruginea on Blood Coagulation
    AU  - Messanh Delagnon Irenee Kueviakoe
    AU  - Sabrina Chris Janiba Sanvee
    AU  - Hezouwe Magnang
    AU  - Komlan Mawubédjro Dossou-Yovo
    AU  - Aboudoulatif Diallo
    AU  - Ahoefa Vovor
    AU  - Kwashie Eklu-Gadegbeku
    Y1  - 2022/05/24
    PY  - 2022
    N1  - https://doi.org/10.11648/j.jdmp.20220802.14
    DO  - 10.11648/j.jdmp.20220802.14
    T2  - Journal of Diseases and Medicinal Plants
    JF  - Journal of Diseases and Medicinal Plants
    JO  - Journal of Diseases and Medicinal Plants
    SP  - 41
    EP  - 45
    PB  - Science Publishing Group
    SN  - 2469-8210
    UR  - https://doi.org/10.11648/j.jdmp.20220802.14
    AB  - Bridelia ferruginea is a plant commonly used in several regions of the world for its various properties. It is offered to certain patients with cerebrovascular accidents. It is with this in mind that this study is undertaken in order to verify whether B. ferruginea has an activity on blood coagulation. Materiel and method: Stem bark of B. ferruginea was collected at Noepe (24 km northwest of Lome). Hydroalcoholic extract (50/50) was obtained by evaporation. Male Wistar rats were used for the tests. Qualitative screening was done according to conventional methods and total phenols were quantified. Some parameters of coagulation (blood platelets, aPTT, PR and fibrinogen) were determined. Oxidative stress was induced by Fe-NTA. Results were analysed by GraphPad®Prism 8.4.2. Analysis of variance (ANOVA) was used to compare several groups. The difference between groups was determined by Tukeyʼs test and considered significant at pResults: Hydroalcoholic extract of B. ferruginea increased aPTT and decreased PR. Compared to the negative control (distilled water), oxidative stress was responsible in vivo for an increase in platelets blood number and PR and a decrease in aPTT and fibrinogen. The hydroalcoholic extract of B. ferruginea caused a decrease in platelets blood number (at low concentration), PR and fibrinogen level and an increase in the platelets blood number (at high concentration) and aPTT. Hydroalcoholic extract of B. ferruginea decreased MDA levels and increased GSH levels in rats subjected to oxidative stress. Conclusion: The present study proved existence of antioxidant and anticoagulant activities of B. ferruginea stem bark. There is a link between oxidative stress and coagulation. Future studies may better elucidate interaction between oxidative stress and coagulation mechanisms involved.
    VL  - 8
    IS  - 2
    ER  - 

    Copy | Download

Author Information
  • Department of Hematology, Faculty of Health Sciences, University of Lome, Lome, Togo

  • Department of Pharmaceuticals Sciences, Faculty of Health Sciences, University of Lome, Lome, Togo

  • Department of Hematology, Faculty of Health Sciences, University of Lome, Lome, Togo

  • Department of Animal Physiology and Pharmacology, Faculty of Sciences, University of Lome, Lome, Togo

  • Department of Pharmaceuticals Sciences, Faculty of Health Sciences, University of Lome, Lome, Togo

  • Department of Hematology, Faculty of Health Sciences, University of Lome, Lome, Togo

  • Department of Animal Physiology and Pharmacology, Faculty of Sciences, University of Lome, Lome, Togo

  • Sections